Estimation of Available Phosphorus in Soil Using the Population of Arbuscular Mycorrhizal Fungi Spores

Machfud Effendy, Bhakti Wisnu Wijayani


Soil microbes, such as arbuscular mycorrhizal fungi (AMF) have the ability to dissolve unavailable phosphorus (P)  and they can be used as an indicator of the P availability in soil. The study was conducted on upland soil  in East Java. The soil  was sampled twice, before and after planting at the harvesting time. The population of AMF spores and soil P availability were observed. The AMF spores were isolated using wet sieving method, decanting, and followed by sucrose density gradient centrifugation. The available P was observed using the Olsen extraction. The numbers of AMF spore was corelated with available P, moreover the numbers of AMF spore was compared to the availabality of P.  The results showed that the total number of AMF spores at six sites were ranged from a little to midle, and the available P ranged from low to high level. All soil site samples had a linear corelation between numbers of AMF spore and  available P in soil.  The greater the number of AMF spore, the higher the available P in soil. It was likely that the availability of P in soil can be predicted by  the population of AMF spores in soil. Therefore, the number of AMF spore can be need as a biological method to predict the available P  in soil and to make a recommendation the use of P fertilizer.


Available P; biological method; correlation; number of AMF spores

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Barea JM. 1991. Vesicular-arbuscular mycorrhizae as modifier of soil fertility. In: Advance in Soil Science by: Springer-Verlag, New York. Vol. 15: 1-40.

Bieleski RL. 1973. Phosphorus pools, phosphate transport and phosphate availability. Am Rev Plant Physiol 24: 225-252.

Bray RH. 1948. Correlation of soil tests with the crop response to added fertilizers and with fertilizer requirement. Diagnostic Techniques for Soils and Crops, Chapter II, American Potash Institute, Washington, DC, pp. 53-86.

Daniels BA and HD Skipper. 1982. Methods for the recovery quantitative estimation of propagules from soil. In: NC Scheck (ed). Methods and Principles of Mycorrhizal Research. American Phytopatology Society, St. Paul, MN, USA, pp. 29-35.

Effendy M, B Prasetijo, Muljadi and W Mindari. 2006. Kajian potensi biofertilizer mikoriza dalam mereduksi kemampuan tanah mengikat P dan implikasinya dalam efisiensi pemberian pupuk P. J Agritek 14: 1074-1088 (in Indonesian).

Effendy M and WW Bhakti. 2008. Studi hifa eksternal CMA dalam memahami fungsinya mengkontribusi serapan P tanaman menggunakan Metode Thin Section. J Trop Soils 13 (3): 241-252 (in Indonesian).

Effendy M. 2010. Produktivitas biologi tanah untuk meningkatkan produktivitas tanah dalam menunjang system pertanian berkelanjutan. Prosiding Seminar Nasional Himpunan Perlindungan Tumbuhan Indonesia. Surabaya 14 April 2010, pp. 107-112 (in Indonesian).

Ezawa T, TR Cavagnaro, SE Smith, FA Smith and R Ohtomo. 2004. Rapid accumulation of polyphosphate in extraradical hyphae of an arbuscular mycorrhizal fungus as revealed by histochemistry and a polyphosphate kinase/luciferase system. New Phytol 161: 387-392.

Gerdeman JW and TH Nicolson, 1963. Spores of mycorrhizal endogone species extracted from soil by wet sieving and decanting. Trans British Mycol Soc 46: 235-244.

O’Keefe DM and DM Sylvia. 1991. Mechanisms of the vesicular-arbuscular mycorrhizal plant growth response. In: DK Arora, B Rai, KG Mukerji and GR Krudsen (eds). Handbook of applied Mycology Vol. 1: Soil and Plants. Marcel Dekker, Inc. New York, pp. 35-53.

Owuzu-Bennoah E and A Wild. 1980. Effects of vesicular-arbuscular mycorrhizae on the size of labile pool of soil phosphate. In : P Mikola (ed). Tropical Mycorrhiza Researh. Clarendon Press Oxford, p. 321.

Prasetya B and M Effendy. 2003. Potensi andisols sebagai sumber isolat mikoriza arbuskula untuk tanaman kentang. J Agritek 11 (2): 2665-2693.

Schachtman DP, RJ Reid and SM Ayling. 1998. Phosphorus uptake by plants: From soil to cell. Plant Physiol 116: 447-453.

Setijono S. 1996. Fosfor dalam tanah (bab IX), Intisari Kesuburan tanah. Penerbit IKIP Malang, pp. 105-118 (in Indonesian).

Stribley DP. 1987. Mineral Nutrition. In: GR Safir (ed). Ecophysiology of Vesicular-arbuscular Mycorrhizae plants. CRC Press Florida, pp 59-70.

Sulaeman, Suparto and Eviati. 2005. Petunjuk Teknis Analisa Kimia Tanah, Tanaman, Air, dan Pupuk. Balai Penelitian TanahBadan Penelitian dan Pengembangan Pertanian Departemen Pertanian, 2005. Accessed on July 8, 2010, 143 pp.

Tinker PB. 1980. Role of Rhizosphere microorganisms in phosphorus uptake by plants. In: FE Khas, EC Sample and EJ Kamprath (eds). The Role of Phosphorus in Agriculture. American Society of Agronomy, Madison, pp. 617-654.



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