Environmental stressors influence the acdS gene abundance in the rhizospheric soil of pineapple plant in Lampung, Indonesia

Dori Kusuma Jaya; Giyanto Giyanto; Novik Nurhidayat; Sarjiya Antonius

doi:10.5400/jts.2024.v29i3.%p

Environmental stressors influence the acdS gene abundance in the rhizospheric soil of pineapple plant in Lampung, Indonesia

Dori Kusuma Jaya, Giyanto Giyanto, Novik Nurhidayat, Sarjiya Antonius

Abstract

Indonesia has faced extensive poor soil quality for years. Anthropogenic, climatic, and plant pathogenic activities are the major contributing factors that degrade the soil’s essential life-supporting system, such as the functional gene pool. One such gene is the acdS, which codes for the synthesis of the ACC (1-aminocyclopropane-1-carboxylate) deaminase that cleaves ACC as an ethylene hormone precursor in the stressed plant growing under stressful condition. A quantitative real-time polymerase chain reaction (qPCR)-based method was applied to assess acdS abundance in the rhizospheric soil in a pineapple plantation area that has been exposed extensively to herbicides, pathogenic Phytophthora spp infections, and flooding. This study reveals that the abundance of acdS in the rhizospheric soil of herbicide-treated and Phytophthora-infected symptomatic plants is relatively lower than that of non-treated one. The highest acdS abundance was found in the Phytophthora-infected soil of asymptomatic plants. However, there was no significant difference in acdS abundance between the flooded rhizospheric soil and the non-treated one. Hopefully, this data provide a better understanding of proper land-use management practices

Keywords

ACC deaminase; acdS gene abundance; stress ethylene; pineapple; qPCR

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DOI: http://dx.doi.org/10.5400/jts.2024.v29i3.%25p